Class I MHC/Peptide Complexes

Table 1. Human and Murine Class I MHC/peptide complexes

All complexes, both human and murine, are prepared with human β2 microglobulin unless listed otherwise.

Human MHC/peptide complexes

A List Tetramers: These reagents are tested on reference T cell lines cell before shipping.

HLA Protein Position Peptide * Complex Type **
HLA-A*0201 EBV BMLF1 259-267 GLCTLVAML BSP
hCMV pp65 495-503 NLVPMVATV BSP
HIV RT 896-904 ILKEPVHGV BSP
influenza M1 58-66 GILGFVFTL BSP
MAGE-10 254-262 GLYDGMEHL BSP
MELAN-A / MART-1 26-35 ELAGIGILTV (39) BSP
NY-ESO-1 157-165 SLLMWITQC BSP
SLLMWITQA BSP
tyrosinase 369-377 YMNGTMSQV BSP

*Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs.

** Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain

B List Tetramers: These reagents are suitable for research purposes.

HLA * Protein Position Peptide ** Complex Type ***
HLA-A*0101 influenza NP 44-52 CTELKLSDY BSP
MAGE-3 168-176 EVDPIGHLY BSP
tyrosinase 146-156 SSDYVIPIGTY BSP
243-251 KCDICTDEY BSP
HLA-A*0201 AFP 158-166 FMNKFIYEI BSP
325-334 GLSPNLNRFL BSP
BK polyomavirus VP1 90-99 STARIPLPNL BSP
108-116 LLMWEAVTV BSP
B-Raf 597-606 LLTEKSRWSV BSP
CAMEL 1-11 MLMAQEALAFL BSP
CEA 605-613 YLSGANLNL BSP
YLSGADLNL BSP
YLSGANLDL BSP
691-699 IMIGVLVGV BSP
694-702 GVLVGVALI BSP
GLLVGVALI BSP
GVLVGVALV BSP
GLLVGVALV BSP
CSPG4 / HMW-MAA 561-569 SLMVILEHT BSP
769-777 ILSNLSFPV BSP
1063-1071 LLFGSIVAV BSP
2238-2246 LILPLLFYL BSP
cytokeratin-18 365-373 ALLNIKVKL BSP
DCT / TRP-2 180-188 SVYDFFVWL BSP
EBV NA-6 284-293 LLDFVRFMGV BSP
EBV BMLF1 259-267 GLATLVAML BSP
fibromodulin 250-259 YMEHNNVYTV BSP
G250 / CA-IX 24-32 QLLLSLLLL BSP
254-262 HLSTAFARV BSP
gp100 / Pmel 17 154-162 KTWGQYWQV BSP
209-217 ITDQVPFSV BSP
IMDQVPFSV BSP
280-288 YLEPGPVTA BSP
YLEPGPVIA BSP
457-466 LLDGTATLRL BSP
476-485 VLYRYGSFSV BSP
hCMV pp65 14-22 VLGPISGHV BSP
hepatitis capsid protein 18-27 FLPSDFFPSV BSP
HER2 / neu 369-377 KIFGSLAFL BSP
HERV 69-77 DLNNFCQKV (44) BSP
HERV-K-Mel MLAVISCAV (40) BSP
HPV16 E7 11-20 YMLDLQPETT BSP
86-93 TLGIVCPI BSP
influenza NA 213-221 CVNGSCFTV BSP
CVNGSCFTI BSP
JC polyomavirus VP1 36-44 SITEVECFL BSP
LAGE-1 86-94 RLLQLHITM BSP
103-111 ELVRRILSR BSP
MAGE-3 112-120 KVAELVHFL BSP
157-166 SLQLVFGIEL BSP
271-279 FLWGPRALV BSP
MAGE-4 230-239 GVYDGREHTV BSP
GVYDGRIHTV BSP
MAGE-C2 336-344 ALKDVEERV BSP
Mdm2 81-88 LLGDLFGV BSP
MELAN-A / MART-1 26-35 EAAGIGILTV BSP
AAAGIGILTV BSP
ALAGIGILTV BSP
VVAGIGILAI BSP
27-35 LAGIGILTV BSP
ALGIGILTV BSP
MOG 210-218 TLFVIVPVL BSP
mouse TERT 545-553 QLLRSFFYI BSP
797-806 SLFDFFLHFL BSP
981-990 YLQVNSQTV BSP
GnT-V (43) VLPDVFIRC BSP
VLPDVFIRCV BSP
NPM-1 (45) 183-191 AIQDLCLAV BSP
AIQDLCVAV BSP
NY-BR-1 158-167 LLSHGAVIEV BSP
960-968 SLSKILDTV BSP
1365-1373 LLKEKNEEI BSP
NY-ESO-1 85-96 SRLLEFYLAMPF BSP
86-94 RLLEFYLAM BSP
108-116 SLAQDAPPL BSP
127-135 TVSGNILTI BSP
157-165 ALLMWITQC BSP
SALMWITQC BSP
SLAMWITQC BSP
SLLAWITQC BSP
SLLMAITQC BSP
SLLMWATQC BSP
SLLMWIAQC BSP
SLLMWITAC BSP
157-166 SLLMWITQCF BSP
157-167 SLLMWITQCFL BSP
157-170 SLLMWITQCFLPV BSP
158-166 LLMWITQCF BSP
158-167 LLMWITQCFL BSP
159-167 LMWITQCFL BSP
161-169 WITQCFLPV BSP
p53 264-272 LLGRNSFEV BSP
P. aeruginosa probable sulfate transporter 125-133 LAGIGILIV BSP
PRAME 300-309 ALYVDSLFFL BSP
proteinase 3 169-177 VLQELNVTV BSP
RHAMM 165-173 ILSLELMKL BSP
Rab-38 49-58 KVLHWDPETV BSP
50-58 VLHWDPETV BSP
SSX2 41-49 KASEKIFYV BSP
KASEKITYV BSP
103-111 RLQGISPKI BSP
ALQGISPKI BSP
ALQGASPKI BSP
ALQGISAKI BSP
ALQGISPAI BSP
SSX4 41-49 KSSEKIVYV BSP
survivin 96-104 LTLGEFLKL BSP
LMLGEFLKL BSP
TERT 540-548 ILAKFLHWL BSP
tyrosinase 1-9 MLLAVLYCL BSP
369-377 YMDGTMSQV BSP
transaldolase 168-176 LLFSFAQAV BSP
vaccinia virus C16/B22 60-68 CLTEYILWV BSP
WT1 126-134 RMFPNAPYL BSP
235-243 CMTWNQMNL BSP
HLA-A*0301 influenza M1 27-35 RLEDVFAGK BSP
influenza NP 265-273 ILRGSVAHK BSP
HLA-A*2301 influenza M1 58-66 GILGFVFTL BSP
HLA-A*2402 HER2 / neu 63-71 TYLPTNASL BSP
HIV nef 135-142 RYPLTFGW BSP
MAGE-4 143-151 NYKRCFPVI BSP
NY-ESO-1 158-166 LLMWITQCF BSP
162-170 ITQCFLPVF BSP
SAGE 715-723 LYATVIHDI BSP
TERT 324-332 VYAETKHFL BSP
tyrosinase 206-214 AFLPWHRLF BSP
WT1 235-243 CMTWNQMNL BSP
417-425 RWPSCQKKF BSP
HLA-A*3101 influenza M1 58-66 GILGFVFTL BSP
HLA-A*6801 gp100 / Pmel 17 182-191 HTMEVTVYHR BSP
MAGE-3 115-123 ELVHFLLLK BSP
HLA-B*0702 hCMV pp65 417-426 TPRVTGGGAM BSP
HIV nef 129-138 TPGPGVRYPL BSP
NY-ESO-1 60-72 APRGPHGGAASGL BSP
98-109 TPMEAELARRSL BSP
98-110 TPMEAELARRSLA BSP
HLA-B*1302 influenza M1 58-66 GILGFVFTL BSP
HLA-B*1801 NY-ESO-1 88-96 LEFYLAMPF BSP
HLA-B*3501 EBV NA-4 488-496 AVLLHEESM BSP
gp100 / Pmel 17 630-638 LPHSSSHWL BSP
hCMV pp65 123-131 IPSINVHHY BSP
HIV Gag-Pol 774-782 NPDIVIYQY BSP
MELAN-A / MART-1 26-35 EAAGIGILTV BSP
EPAGIGILTV BSP
EAAGIGILTY BSP
EPAGIGILTY BSP
NY-ESO-1 92-100 LAMPFATPM BSP
92-104 LAMPFATPMEAEL BSP
93-104 AMPFATPMEAEL BSP
94-102 MPFATPMEA BSP
94-104 MPFATPMEAEL BSP
96-104 FATPMEAEL BSP
116-123 LPVPGVLL BSP
tyrosinase 312-320 LPSSADVEF BSP
HLA-B*3503 influenza M1 58-66 GILGFVFTL BSP
NY-ESO-1 92-100 LAMPFATPM BSP
92-104 LAMPFATPMEAEL BSP
93-104 AMPFATPMEAEL BSP
94-102 MPFATPMEA BSP
94-104 MPFATPMEAEL BSP
HLA-Cw*0304 importin-α2 / karyopherin 204-212 GAVDPLLAL BSP
NY-ESO-1 92-100 LAMPFATPM BSP
92-104 LAMPFATPMEAEL BSP
96-104 FATPMEAEL BSP
HLA-Cw*0702 influenza M1 58-66 GILGFVFTL BSP

*Also available:

  • HLA-A*0201 mutants :D227K, T228A, D227K T228A, T233A, A245V
  • HLA-A*1101
  • HLA-A*3001
  • HLA-A*3004
  • HLA-B*0801
  • HLA-B*2705
  • HLA-B*5101
  • HLA-Cw*0303
  • HLA-Cw*0401
  • HLA-Cw*0602
  • HLA-Cw*1402

**Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs.

*** Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain.

C List Tetramers: These reagents are suitable for special applications.

HLA Protein Position Peptide * Complex Type **
HLA-A*0201 CD8 binding-deficient tetramers
(4, 25, 26)
CEA 694-702 GVLVGVALI D227K T228A
GLLVGVALI D227K T228A
GVLVGVALV D227K T228A
GLLVGVALV D227K T228A
EBV IE63 259-267 GLCTLVAML A245V BSP
D227K T228A
gp100 / Pmel 17 209-217 ITDQVPFSV D227K T228A
IMDQVPFSV D227K T228A
hCMV pp65 495-503 NLVPMVATV A245V BSP
D227K T228A
HER2 / neu 369-377 KIFGSLAFL D227K T228A
influenza M1 58-66 GILGFVFTL A245V BSP
D227K T228A
MAGE-10 254-262 GLYDGMEHL D227K BSP
T228A BSP
A245V BSP
D227K T228A
Mdm2 81-88 LLGDLFGV D227K T228A
MELAN-A / MART-1 26-35 EAAGIGILTV D227K T228A
ELAGIGILTV D227K BSP
T228A BSP
A245V BSP
D227K T228A
NY-ESO-1 157-165 SLLMWITQC D227K BSP
T228A BSP
A245V BSP
D227K T228A
SLLMWITQA D227K T228A
p53 264-272 LLGRNSFEV D227K T228A
proteinase 3 169-177 VLQELNVTV D227K T228A
RHAMM 165-173 ILSLELMKL D227K T228A
SSX2 41-49 KASEKIFYV D227K BSP
T228A BSP
A245V BSP
D227K T228A
KASEKITYV D227K T228A
WT1 126-134 RMFPNAPYL D227K T228A
HLA-A*0201 α1 α2 H-2Kb α3 *** DCT / TRP-2 180-188 SVYDFFVWL BSP mβ2m
HERV 65-74 SILQDLNNFV (44) BSP
influenza M1 58-66 GILGFVFTL BSP
MELAN-A / MART-1 26-35 ELAGIGILTV BSP
ELAGIGILIV BSP
NY-ESO-1 157-165 SLLMWITQC BSP
157-166 SLLMWITQCF BSP
157-167 SLLMWITQCFL BSP
158-166 LLMWITQCF BSP
158-167 LLMWITQCFL BSP
159-167 LMWITQCFL BSP
161-169 WITQCFLPV BSP
Reversible HLA-A*0201
(11, 22)
EBV IE63 259-267 GLCTLVAML
MELAN-A / MART-1 26-35 ELAGIGILTV
NY-ESO-1 157-165 SLLMWITQA

*Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs.

**Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain; mβ2m, mouse microglobulin.

*** These are chimeric complexes whose heavy chains are in part human and in part murine.

Murine MHC/peptide complexes

HLA Protein Position Peptide * Complex Type **
H-2Kd HLA-A2 194-203 RYLENGKETL BSP
HLA-Cw3 194-203 RYLKNGKETL BSP
BSP mβ2m
D227K BSP ***
HER2 / neu 63-71 TYLPTNASL BSP
influenza HA 204-212 LYQNVGTYV BSP
BSP mβ2m
mouse ERK2 136-144 QYIHSANVL BSP
BSP mβ2m
P. berghei CS 252-260 SYIPSAEKI BSP
BSP mβ2m
SYIPSAEK(ATTO)I BSP
SYIPSAEK(ABA)I BSP
BSP mβ2m
D227K BSP ***
Dap[Cy5]-YIPSAEK(ABA)I BSP
SYILSAEK(ABA)I BSP
SYIASAEK(ABA)I BSP
H-2Db GFP 117-125 DTLVNRIEL BSP
118-125 TLVNRIEL BSP
gp100 / Pmel 17 25-33 KVPRNQDWL BSP
HPV16 E7 49-57 RAHYNIVTF BSP
HPV16 L1 165-173 AGVDNRECI BSP
influenza NP (1968) 366-374 ASNENMDAM BSP
D227K BSP ***
influenza NP (1976) 366-374 ASNENMETM BSP
D227K BSP ***
influenza PA 224-233 SSLENFRAYV BSP
influenza PB1 62-70 LSLRNPILV BSP
LCMV GPC 33-41 KAVYNFATC BSP
BSP mβ2m
KAVYNFATA BSP
D227K BSP ***
Q226A D227K BSP ***
276-286 SGVENPGGYCL BSP
D227K BSP ***
LCMV NP 396-404 FQPQNGQFI BSP
mouse HY 738-746 KCSRNRQYL BSP
D227K BSP ***
mouse gp100 25-33 EGSRNQDWL BSP
mouse Spas-1 H8 244-252 STHVNHLHC BSP
NY-ESO-1 86-94 RLLEFYLAM BSP
H-2Dk MPyV MT 389-396 RRLGRTLL BSP mβ2m
H-2Dd HIV env 309-318 RGPGRAFVTI BSP
BSP mβ2m
NY-ESO-1 81-88 RGPESRLL BSP
H-2Kb chicken ovalbumin 258-265 SIINFEKL BSP mβ2m
DCT / TRP-2 180-188 SVYDFFVWL BSP
E. coli β-gal 97-104 DAPIYTNV BSP
H4 87-93 VVYAFKR BSP
influenza PB1 703-711 SSYRRPVGI BSP
LCMV GPC 34-41 AVYNFATC BSP
LCMV NP 205-212 YTVKYPNL BSP
MHV S 598-605 RCQIFANI BSP
NY-ESO-1 87-94 LLEFYLAM BSP
SV40 LT 404-411 VVYDFLKC BSP
H-2Ld MLV gp70 AH-1 138-147 SPSYVYHQF (41) BSP
H-2QAI mouse H2-L / Qdm 3-11 AMAPRTLLL
TL T3b TL (42) BSP

* Amino acid residues which have been modified with respect to the native sequence are shown in red in peptide analogs. Labels in blue: ABA, 4-azidobenzoic acid; ATTO, low molecular weight fluorescent dyes (http://www.attotech.com/) for flow cytometry analysis. Label in magenta: Dap, diamino-propionic acid.

**Abbreviations: BSP, biotinylation sequence peptide (LHHILDAQKMVWNHR) added C-terminally at the α3 domain; mβ2m, mouse microglobulin.

*** CD8 binding-deficient tetramers.